THE COLLEGE OF PATHOLOGISTS

OF SRI LANKA

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Workshop on Basics of Immunohistochemistry

Time

Topic

Resource Person

Wednesday 12th June 2019

8.30-8.55am

Registration

 

8.55-9.00am

Welcome remarks

 

9.00-9.30am

Immunohistochemical staining and staining protocol; the scientific basis

Dr Eranga Siriweera

9.30-10.00am

Antibodies-polyclonal vs monoclonal, affinity, cross-reactivity, reaction rates, stability and handling of antibodies

Ms. Hazel Chambers-Smith

10.00-10.30am

Possible pit falls at each step in immunohistochemical staining

Ms. Hazel Chambers-Smith

10.30-10.50am

TEA

10.50-11.20am

Problems faced with IHC in the Sri Lankan setting

Prof. Neelakanthi Ratnatunge

11.20am-12.00

Selection, validation and optimization of a new antibody

Ms. Hazel Chambers-Smith

12.00 -12.20pm

Verification of new lots and expired antibodies

Ms. Hazel Chambers-Smith

12.20-12.50pm

Controls in immunohistochemistry

Dr. Cherine Sosai

12.50-1.40pm

LUNCH

1.40-2.10pm

Calculations for immunohistochemistry

Ms. Hazel Chambers-Smith

2.10- 2.30pm

Validation of ready to use antibodies

Ms. Hazel Chambers-Smith

2.30-3.00pm

Introduction to automated platform for Immunohistochemistry, Manual staining vs automated staining

Ms. Hazel Chambers-Smith

3.00-3.30pm

TEA

 

 

Thursday 13th June 2019

9.00-9.30am

Maintenance of Immunohistochemistry auto-stainers

Ms. Hazel Chambers-Smith

9.30-10.00am

Addressing no staining, high background and non-specific staining; troubleshooting

Ms. Hazel Chambers-Smith

10.00-10.30am

Validation of a new detection system and instrumentation

Ms. Hazel Chambers-Smith

10.30-11.00am

TEA

11.00-11.40am

Ordering antibodies and other consumables for immunohistochemistry, a practical approach

Ms. Hazel Chambers-Smith

11.40am-12.00

Currently available autostainer platforms in Sri Lanka

 

12.00-12.10pm

Closing remarks

 

 

 

 


 




 

 



 

 

Pathology is the medical specialty concerned with the study of the nature and causes of diseases. The College of Pathologists of Sri Lanka is the leading professional organization representing this field in the country. On this website you can access the details about our college and its activities

 

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College of Pathologists of Sri Lanka,

No.6, Wijerama Mawatha,
Colombo 7

Tel : +94 112 685 088
Email : colpathsl@yahoo.com